The Structural Variety of DNA-DPPC-Divalent Metal Cation Aggregates: SAXD and SANS Study

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DOIResolve DOI: http://doi.org/10.1140/epjst/e2009-00957-0
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TypeArticle
Journal titleThe European Physical Journal Special Topics
Volume167
Pages191197; # of pages: 7
AbstractWe examine the structure of aggregates formed due to DNA interaction with dipalmitoylphosphatidylcholine (DPPC) in presence of Ca2+ and Zn2+ using small-angle synchrotron X-ray diffraction (SAXD) and neutron scattering (SANS). SAXD shows structural heterogeneity as a function of the cation concentration and temperature: At low cation concentration (∼1mM), aggregates show two DPPC phases, one with a lateral segregation of DNA and cation, while higher cation concentration improves the DNA packing and the condensed lamellar phase is observed in DNA + DPPC + 20mMion2+ aggregates. The SANS detected the dissolution of the condensed lamellar phase into unilamellar DPPC + Zn2+ vesicles due to gel → liquid-crystal phase transition in DNA + DPPC + 20mM Zn2+ aggregates with the short fragmented salmon sperm DNA.
Publication date
LanguageEnglish
AffiliationNRC Canadian Neutron Beam Centre; National Research Council Canada
Peer reviewedYes
NPARC number14829324
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Record identifierca0d3cbf-d6e6-434c-b972-b6556712b6fe
Record created2011-02-15
Record modified2016-05-09
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