Adjuvant potential of archaeal synthetic glycolipid mimetics critically depends on the glyco head group structure

Download
  1. Get@NRC: Adjuvant potential of archaeal synthetic glycolipid mimetics critically depends on the glyco head group structure (Opens in a new window)
AuthorSearch for: ; Search for: ; Search for: ; Search for:
TypeArticle
Journal titleGlycobiology
Volume18
Issue7
Pages559565; # of pages: 7
SubjectAdjuvants,Immunologic; Animals; ANTIGEN; Antigens; Archaea; Canada; carbohydrate; CD8-Positive T-Lymphocytes; cell; chemical synthesis; chemistry; DISACCHARIDE; Disaccharides; drug effects; Female; Glucose; Glyceryl Ethers; glycolipid; Glycolipids; Halobacterium salinarum; immunology; lipid; lipid vesicles; Lipids; Mice; Mice,Inbred C57BL; Molecular Mimicry; pharmacology; POTENT; POTENTIAL; SERIES; stability; structure; SUBUNIT; synthetic; toxicity; Vaccines
AbstractSubunit vaccines capable of providing protective immunity against the intracellular pathogens and cancers that kill millions of people annually require an adjuvant capable of directing a sufficiently potent cytotoxic T lymphocyte response to purified antigens, without toxicity issues. Archaeosome lipid vesicles, prepared from isoprenoid lipids extracted from archaea, are one such adjuvant in development. Here, the stability of an archaeal core lipid 2,3-di-O-phytanyl-sn-glycerol (archaeol) is used to advantage to synthesize a series of disaccharide archaeols and show that subtle variations in the carbohydrate head group alters the type and potency of immune responses mounted in a mammal. Critically, a glycosylarchaeol was required to elicit high cytotoxic CD8(+) T cell activity, with highest responses to the antigen entrapped in archaeosomes containing disaccharides of glucose in beta- or alpha1-6 linkage (beta-gentiobiose, beta-isomaltose), or of beta-lactose. This first study on synthetic archaeal lipid adjuvants reveals potential for this class of regulatory friendly, easily scalable, inexpensive, and potent glyco-adjuvant
Publication date
LanguageEnglish
AffiliationNRC Institute for Biological Sciences; National Research Council Canada
Peer reviewedNo
NRC numberSPROTT2008
NPARC number9362912
Export citationExport as RIS
Report a correctionReport a correction
Record identifierca223567-714a-4bab-a68f-a70812928e35
Record created2009-07-10
Record modified2016-05-09
Bookmark and share
  • Share this page with Facebook (Opens in a new window)
  • Share this page with Twitter (Opens in a new window)
  • Share this page with Google+ (Opens in a new window)
  • Share this page with Delicious (Opens in a new window)