Production of specifically labeled compounds by Methanobacterium espanolae grown on H 2 -CO 2 plus [ 13 C] acetate

Download
  1. Get@NRC: Production of specifically labeled compounds by Methanobacterium espanolae grown on H 2 -CO 2 plus [ 13 C] acetate (Opens in a new window)
AuthorSearch for: ; Search for: ; Search for:
TypeArticle
Journal titleApplied and Environmental Microbiology
Volume59
IssueAPR
Pages10991103; # of pages: 5
AbstractMethanobacterium espanolae, an acidiphilic methanogen, required acetate for maximal growth on H2-CO2. In the presence of 5 to 15 mM acetate, at a growth pH of 5.5, the μmax was 0.05 h-1. M. espanolae consumed 12.3 mM acetate during 96 h of incubation at 35°C with shaking at 100 rpm. At initial acetate levels of 2.5 to 10.0 mM, the amount of biomass produced was dependent on the amount of acetate in the medium. 13C nuclear magnetic resonance spectra of protein hydrolysates obtained from cultures grown on [1-13C]- or [2-13C]acetate indicated that an incomplete tricarboxylic acid pathway, operating in the reductive direction, was functional in this methanogen. The amino acids were labeled with a very high degree of specificity and at greater than 90% enrichment levels. Less than 2% label randomization occurred between positions primarily labeled from either the carboxyl or methyl group of acetate, and very little label was transferred to positions primarily labeled from CO2. The labeling pattern of carbohydrates was typical for glucogenesis from pyruvate. This methanogen, by virtue of the properties described above and its ability to incorporate all of the available acetate (10 mM or lower) from the growth medium, has advantages over other microorganisms for use in the production of specifically labeled compounds.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada; NRC Institute for Biological Sciences; NRC Biotechnology Research Institute
Peer reviewedNo
NRC numberPATEL1993
34310
NPARC number9388126
Export citationExport as RIS
Report a correctionReport a correction
Record identifierca9146fc-8f0b-4ac1-a49e-6ce359e5ac39
Record created2009-07-10
Record modified2016-05-09
Bookmark and share
  • Share this page with Facebook (Opens in a new window)
  • Share this page with Twitter (Opens in a new window)
  • Share this page with Google+ (Opens in a new window)
  • Share this page with Delicious (Opens in a new window)