Picoamperometric detection of glucose at ultrasmall platinum based biosensors: Preparation and characterization

DOIResolve DOI: http://doi.org/10.1021/ac026438u
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TypeArticle
Volume75
Issue14
Pages33083315; # of pages: 8
Subjectenv; Glucose
AbstractA simple method is described for the construction of a glucose biosensor with good reproducibility. After electrochemical etching, the sensing tip of an etched platinum microelectrode was insulated using a synthetic rubber dip coating. The insulating layer was then heat-cured, leading to a small exposed area at the very end of the etched Pt tip, as confirmed by scanning electron microscopy. Phenol and 2-allylphenol were electropolymerized to form an extra insulating layer that effectively retained glucose oxidase (GOX) on the sensing tip of the electrode. On the basis of cyclic voltammetry measurements, the apparent radius of the biosensor tip was estimated to be between 10 and 500 nm, depending on GOX loading. With operational and storage stabilities over 3 weeks, the glucose biosensor prepared using optimal GOX concentration (10 mg/mL) exhibited a picoamperometric current response within ~2 s and a detection limit of 20 M with excellent reproducibility.
Publication date
AffiliationNRC Biotechnology Research Institute; National Research Council Canada
Peer reviewedNo
NRC number45931
NPARC number3540251
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Record identifierca943732-6521-4031-8a19-c29396a5101a
Record created2009-03-01
Record modified2016-05-09
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