Paralytic shellfish toxins, including deoxydecarbamoyl-STX, in wild-caught Tasmanian abalone (Haliotis rubra)

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DOIResolve DOI: http://doi.org/10.1016/j.toxicon.2014.08.058
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TypeArticle
Journal titleToxicon
ISSN0041-0101
Volume90
Pages213225; # of pages: 13
Subjectmarine toxin; animal; Australia; gastropod; isolation and purification; liquid chromatography; metabolism; standard; tandem mass spectrometry; Animals; Chromatography, Liquid; Gastropoda; Marine Toxins; Reference Standards; Tandem Mass Spectrometry; Tasmania
AbstractFor the first time wild-caught Tasmanian abalone, Haliotis rubra, have been reported to contain paralytic shellfish toxins (PSTs). This observation followed blooms of the toxic dinoflagellate Gymnodinium catenatum. No illnesses were reported, but harvesting restrictions were enforced in commercial areas. Abalone were assayed using HPLC-FLD methodology based on AOAC official method 2005.06. An uncommon congener, deoxydecarbamoyl-STX (doSTX), was observed in addition to regulated PSTs as unassigned chromatographic peaks. A quantitative reference material was prepared from contaminated Tasmanian abalone viscera and ampouled at 54.2 μmol/L. The LD50 of doSTX via intraperitoneal injection was 1069 nmol/kg (95% confidence limits 983-1100 nmol/kg), indicating it is nearly 40 times less toxic than STX. A toxicity equivalence factor of 0.042 was generated using the mouse bioassay. Levels of PSTs varied among individuals from the same site, although the toxin profile remained relatively consistent. In the foot tissue, STX, decarbamoyl-STX and doSTX were identified. On a molar basis doSTX was the dominant congener in both foot and viscera samples. The viscera toxin profile was more complex, with other less toxic PST congeners observed and was similar to mussels from the same site. This finding implicates localised dinoflagellate blooms as the PST source in Tasmanian abalone.
Publication date
PublisherElsevier
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); Measurement Science and Standards (MSS-SME)
Peer reviewedYes
NPARC number21275449
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Record identifiercc4b617f-0c4e-40a1-925c-84d6ea651267
Record created2015-07-14
Record modified2016-05-09
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