Lipid reorganization in biological membranes. A study by fourier transform infrared difference spectroscopy

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DOIResolve DOI: http://doi.org/10.1016/0005-2736(79)90123-8
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TypeArticle
Journal titleBiochimica et Biophysica Acta (BBA) - Biomembranes
ISSN0005-2736
Volume550
Issue1
Pages145149
SubjectLipid reorganization; Fourier transform; Phase transition; (Infrared difference)
AbstractThe first application of infrared difference spectroscopy to the study of a natural biological membrane is described. Perdeuterated palmitic acid was incorporated biosynthetically into the lipids of the plasma membrane of Acholeplasma laidlawii and the temperature-induced structural rearrangement of the endogenous lipids monitored via their C2H vibrational modes. Changes in infrared parameters were studied between 0 and 50°C and contrasted with those occurring in the model membrane system of 1,2-diperdeuteropalmitoyl-sn-glycero-3-phosphocholine. The phase transition of the biomembrane occurs over a 20°C range with the temperature of the maximum rate of change of absorbance coinciding with that of the sharp phase transition of the model membrane.
Publication date
PublisherElsevier B.V.
LanguageEnglish
AffiliationNational Research Council Canada
Peer reviewedYes
NRC number16933
NPARC number23001278
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Record identifiercc74a748-9dcf-462c-94a4-753b91443ca5
Record created2017-01-13
Record modified2017-01-13
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