Glycosylation of Pseudomonas aeruginosa strain Pa5196 type IV pilins with mycobacterium-like alpha-1,5-linked d-Araf oligosaccharides: J.Bacteriol.

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TypeArticle
Journal titleJ Bacteriol.
Volume189
Issue1
Pages151159; # of pages: 9
SubjectACID; amino acid; Amino Acid Sequence; AMINO-ACID; AMINO-ACID-SEQUENCE; analogs & derivatives; Antibodies; antibody; Arabinose; Bacteria; biosynthesis; Canada; cell; Cell Wall; chemistry; ELUCIDATION; Fimbriae Proteins; genetics; Glycosylation; Gram Negative Bacteria; Gram-Negative Bacteria; LIPOPOLYSACCHARIDE; Lipopolysaccharides; MAGNETIC; Magnetic Resonance Spectroscopy; MAGNETIC-RESONANCE; mass spectrometry; MASS-SPECTROMETRY; metabolism; MODEL; Molecular Sequence Data; MUTANT; Mycobacterium; NUCLEAR; Nuclear Magnetic Resonance; nuclear magnetic resonance spectroscopy; NUCLEAR-MAGNETIC-RESONANCE; O antigen; O-antigen; oligosaccharide; Oligosaccharides; PATHWAY; PATHWAYS; Polymers; Polysaccharides; Pseudomonas; Pseudomonas aeruginosa; PSEUDOMONAS-AERUGINOSA; RESONANCE; SEQUENCE; SPECTROSCOPY; STRAIN; STRAINS; STRUCTURAL; SUBUNIT; sugar; surface; SYSTEM; UNIT; Virulence
AbstractPseudomonas aeruginosa is a gram-negative bacterium that uses polar type IV pili for adherence to various materials and for rapid colonization of surfaces via twitching motility. Within the P. aeruginosa species, five distinct alleles encoding variants of the structural subunit PilA varying in amino acid sequence, length, and presence of posttranslational modifications have been identified. In this work, a combination of mass spectrometry and nuclear magnetic resonance spectroscopy was used to identify a novel glycan modification on the pilins of the group IV strain Pa5196. Group IV pilins continued to be modified in a lipopolysaccharide (wbpM) mutant of Pa5196, showing that, unlike group I strains, the pilins of group IV are not modified with the O-antigen unit of the background strain. Instead, the pilin glycan was determined to be an unusual homo-oligomer of alpha-1,5-linked d-arabinofuranose (d-Araf). This sugar is uncommon in prokaryotes, occurring mainly in the cell wall arabinogalactan and lipoarabinomannan (LAM) polymers of mycobacteria, including Mycobacterium tuberculosis and Mycobacterium leprae. Antibodies raised against M. tuberculosis LAM specifically identified the glycosylated pilins from Pa5196, confirming that the glycan is antigenically, as well as chemically, identical to those of Mycobacterium. P. aeruginosa Pa5196, a rapidly growing strain of low virulence that expresses large amounts of glycosylated type IV pilins on its surface, represents a genetically tractable model system for elucidation of alternate pathways for biosynthesis of d-Araf and its polymerization into mycobacterium-like alpha-1,5-linked oligosaccharides
Publication date
LanguageEnglish
AffiliationNRC Institute for Biological Sciences; National Research Council Canada
Peer reviewedNo
NRC numberVOISIN2007
NPARC number9370305
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Record identifiercc82b30d-881d-4eda-ab14-d31730f3c65a
Record created2009-07-10
Record modified2016-05-09
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