Comprehensive hormone profiling in developing Arabidopsis seeds: examination of the site of abscisic acid biosynthesis, abscisic acid transport and hormone interactions

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DOIResolve DOI: http://doi.org/10.1093/pcp/pcq158
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TypeArticle
Journal titlePlant & Cell Physiology
Volume51
Issue12
Pages19882001; # of pages: 14
Subjectabscisic acid; ABA; dormancy; hormone interaction; liquid chromatography; electrospray; ionization-tandem; mass spectrometry; LC-ESI-MS/MS; seed development; seed maturation
AbstractAbscisic acid (ABA) plays important roles in many aspects of seed development, including accumulation of storage compounds, acquisition of desiccation tolerance, induction of seed dormancy, and suppression of precocious germination. Quantification of ABA in the F1 and F2 populations originated from crosses between wild type and an ABA-deficient mutant aba2-2 demonstrated that ABA was synthesized in both maternal and zygotic tissues during seed development. In the absence of zygotic ABA, ABA synthesized in maternal tissues was translocated into the embryos and partially induced seed dormancy. We also analyzed the levels of ABA metabolites, gibberellins, indole-3-acetic acid (IAA), cytokinins, jasmonates and salicylic acid (SA) in the developing seeds of wild type and aba2-2. ABA metabolites accumulated differentially in the silique and seed tissues during development. Endogenous levels of SA were elevated in aba2-2 in the later developmental stages, whereas that of IAA was reduced compared to wild type. These data suggest that ABA metabolism depends on developmental stages and tissues, and that ABA interacts with other hormones to regulate seed developmental processes.
Publication date
LanguageEnglish
AffiliationNRC Plant Biotechnology Institute; National Research Council Canada
Peer reviewedYes
NRC number50174
NPARC number18906334
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Record identifiercd30ba48-110e-42a6-95e2-76f4b7ce7782
Record created2012-04-02
Record modified2016-05-09
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