Single-nucleotide polymorphism identification and genotyping in Camelina sativa

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DOIResolve DOI: http://doi.org/10.1007/s11032-015-0224-6
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TypeArticle
Journal titleMolecular Breeding
ISSN1380-3743
Volume35
Issue1
Article number35
SubjectCamelina sativa; reduced representation; SNP; Genetic mapping; Diversity; Polyploidy
AbstractCamelina sativa, a largely relict crop, has recently returned to interest due to its potential as an industrial oilseed. Molecular markers are key tools that will allow C. sativa to benefit from modern breeding approaches. Two complementary methodologies, capture of 3′ cDNA tags and genomic reduced-representation libraries, both of which exploited second generation sequencing platforms, were used to develop a low density (768) Illumina GoldenGate single nucleotide polymorphism (SNP) array. The array allowed 533 SNP loci to be genetically mapped in a recombinant inbred population of C. sativa. Alignment of the SNP loci to the C. sativa genome identified the underlying sequenced regions that would delimit potential candidate genes in any mapping project. In addition, the SNP array was used to assess genetic variation among a collection of 175 accessions of C. sativa, identifying two sub-populations, yet low overall gene diversity. The SNP loci will provide useful tools for future crop improvement of C. sativa.
Publication date
PublisherSpringer International Publishing
LanguageEnglish
AffiliationNational Research Council Canada; Aquatic and Crop Resource Development
Peer reviewedYes
NPARC number21277008
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Record identifiercdcea137-bb3e-4feb-80d2-0715e3515733
Record created2015-11-10
Record modified2016-11-15
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