Exploration of attenuated total reflectance mid-infrared spectroscopy and multivariate calibration to measure immunoglobulin G in human sera

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DOIResolve DOI: http://doi.org/10.1016/j.talanta.2015.04.010
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TypeArticle
Journal titleTalanta
ISSN0039-9140
Volume142
Pages110119; # of pages: 10
SubjectBody fluids; Calibration; Correlation methods; Infrared spectroscopy; Reflection; Statistics; ATR-Infrared spectroscopy; Attenuated total reflectance; Immunoglobulin; Partial least squares regression; Pearson correlation coefficients; Relative standard deviations; Umbilical cords; Venous; Least squares approximations
AbstractImmunoglobulin G (IgG) is crucial for the protection of the host from invasive pathogens. Due to its importance for human health, tools that enable the monitoring of IgG levels are highly desired. Consequently there is a need for methods to determine the IgG concentration that are simple, rapid, and inexpensive. This work explored the potential of attenuated total reflectance (ATR) infrared spectroscopy as a method to determine IgG concentrations in human serum samples. Venous blood samples were collected from adults and children, and from the umbilical cord of newborns. The serum was harvested and tested using ATR infrared spectroscopy. Partial least squares (PLS) regression provided the basis to develop the new analytical methods. Three PLS calibrations were determined: one for the combined set of the venous and umbilical cord serum samples, the second for only the umbilical cord samples, and the third for only the venous samples. The number of PLS factors was chosen by critical evaluation of Monte Carlo-based cross validation results. The predictive performance for each PLS calibration was evaluated using the Pearson correlation coefficient, scatter plot and Bland-Altman plot, and percent deviations for independent prediction sets. The repeatability was evaluated by standard deviation and relative standard deviation. The results showed that ATR infrared spectroscopy is potentially a simple, quick, and inexpensive method to measure IgG concentrations in human serum samples. The results also showed that it is possible to build a united calibration curve for the umbilical cord and the venous samples. © 2015 Elsevier B.V. All rights reserved.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); Medical Devices
Peer reviewedYes
NPARC number21275689
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Record identifierce53728c-5ac0-47dd-9e10-2f0e56c51afb
Record created2015-07-14
Record modified2016-05-09
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