Nested PCR protocol for the rapid detection of Escherichia coli in potable water

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DOIResolve DOI: http://doi.org/10.1139/m96-110
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TypeArticle
Journal titleCanadian Journal of Microbiology
Volume42
Issue8
Pages862866; # of pages: 5
Subjectenv; nested PCR; sensitive; detection; potable water
AbstractA rapid and sensitive method for the detection of low levels of bacteria in potable water was developed. The fecal indicator bacterium Escherichia coli was used as the test organism in a filtration concentration-nested polymerase chain reaction (PCR) protocol, combined with ethidium bromide visualization of PCR products. Two sets of primers were designed from the E. coli specific β-glucuronidase gene (uidA), the primary pair producing a 486-bp fragment that was used as template for the nested primer pair delineating a 186-bp fragment. This protocol can detect 1-10 bacterial cells/50 mL water sample within 6-8 h, in contrast to traditional culturing or Southern hybridization methods which require 2-3 days for results.
Publication date
LanguageEnglish
AffiliationNRC Biotechnology Research Institute; National Research Council Canada
Peer reviewedNo
NRC number34557
NPARC number3539699
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Record identifierce75604f-3a25-4e60-a76e-8231c13616b6
Record created2009-03-01
Record modified2016-05-09
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