Analysis of Helicobacter pylori isolates from Chile: occurrence of selective type 1 Lewis b antigen expression in lipopolysaccharide: J.Med.Microbiol.

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TypeArticle
Journal titleJournal of medical microbiology
Volume57
IssuePt 5
Pages585591; # of pages: 7
SubjectAdolescent; Adult; Aged; Aged,80 and over; analysis; Antibodies; antibody; ANTIGEN; Antigens; Antigens,CD15; Blood; blood group; Canada; Chile; Electrophoresis; epidemiology; epitope; Epitopes; EXPRESSION; Female; Gastrointestinal Diseases; Gene Expression Regulation,Bacterial; genetics; Helicobacter Infections; Helicobacter pylori; Humans; immunology; Lewis; Lewis Blood Group System; Lewis Blood-Group System; LIPOPOLYSACCHARIDE; Lipopolysaccharides; LPS; Male; metabolism; Microbiology; Middle Aged; oligosaccharide; Oligosaccharides; physiology; selective; STRAIN; STRAINS; structure; SYSTEM
AbstractPrevious studies have shown that the LPS of Helicobacter pylori isolated from North American and European hosts predominantly expresses type 2 Lewis x (Le(x)) and Le(y) epitopes, whilst the LPS from Asian strains has the capacity to express type 1 Le(a) and Le(b) structures. The aim of this study was to evaluate the expression of Le antigens and the cytotoxin-associated antigen (CagA) by H. pylori isolates from Chile. A total of 38 isolates were screened. The expression of Le antigens and CagA was determined by whole-cell indirect ELISA, using commercially available monoclonal anti-Le and polyclonal anti-CagA antibodies. LPS profiles of H. pylori isolates were assessed by gel electrophoresis and Western blotting. Expression of Le(x) and/or Le(y) epitopes was confirmed in 32/38 isolates (84 %), whilst 9/38 isolates (24 %) expressed type 1 Le(b) blood group determinants, in addition to type 2 Le(x) and Le(y) structures. Six strains (16 %) were non-typeable. The majority of H. pylori strains examined were CagA-positive (83.3 %)
Publication date
LanguageEnglish
AffiliationNRC Institute for Biological Sciences; National Research Council Canada
Peer reviewedNo
NRC numberALTMAN2008
NPARC number9369974
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Record identifiercf589149-4fd8-4e98-89ba-fb88600f219f
Record created2009-07-10
Record modified2016-05-09
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