Carbohydrate analysis and serological classification of typical and atypical isolates of Aeromonas salmonicida: a rationale for the lipopolysaccharide-based classification of A. salmonicida

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TypeArticle
Journal titleFish.Shellfish.Immunol.
Volume23
Issue5
Pages10951106; # of pages: 12
SubjectAeromonas; analysis; bacterial; Canada; capillary; carbohydrate; cell; Cell Membrane; CELLS; chemical; classification; COMPONENT; COMPONENTS; Cross Reactions; DIFFERENCE; ENVELOPE; Immunoblotting; LED; LIPOPOLYSACCHARIDE; LPS; membrane; O-chain; POLYSACCHARIDE; polysaccharide structure; STRAIN; STRAINS; STRUCTURAL; structure; SYSTEM; technique
AbstractThe cell envelope of Aeromonas salmonicida contains a lipopolysaccharide (LPS) essential for the physical integrity and functioning of bacterial cell membrane. Using a recently developed in-source fragmentation technique, we screened 39 typical and atypical isolates of A. salmonicida and established their O-chain polysaccharide structure by capillary electrophoresis-mass spectrometry (CE-MS), compositional and linkage analyses and comparison to the previously determined O-chain polysaccharide structure of A. salmonicida strain A449. These studies have demonstrated that A. salmonicida isolates fall into three distinct structural types, types A-C, based on chemical structures of their respective O-chain polysaccharide components. Subsequent immunoblotting and serological studies with salmon polyclonal antisera produced to formalin-fixed cells of A. salmonicida strains A449, N4705 and 33659 representing three structural types A-C revealed that variations in the O-chain polysaccharide structure have led to significant serological differences between strains belonging to type A and non-type A, where non-type A species include chemically separated structural types B and C. Due to the presence of common antigenic determinants shared by their respective O-chain polysaccharide components, serological cross-reactions were observed between A. salmonicida strains belonging to structural types B and C. These findings suggest the possibility of developing LPS-based classification system of A. salmonicida sub-species consisting of two serologically distinct types, type A and non-type A
Publication date
LanguageEnglish
AffiliationNRC Institute for Biological Sciences; National Research Council Canada
Peer reviewedNo
NRC numberWANG2007A
NPARC number9358338
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Record identifiercfac197f-da95-4e6f-b545-4ff97b6e13ae
Record created2009-07-10
Record modified2016-05-09
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