Structural studies of the O-antigenic polysaccharide of Fusobacterium necrophorum

  1. Get@NRC: Structural studies of the O-antigenic polysaccharide of Fusobacterium necrophorum (Opens in a new window)
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Journal titleEuropean Journal of Biochemistry
Pages801809; # of pages: 9
Subject13C; 1H; 31P; abscess; ACID; Animals; AQUEOUS; bacterial; carbohydrate; Carbohydrate Conformation; Carbohydrate Sequence; Cattle; chemical; CHEMICAL METHODS; chemistry; COMPONENT; CONFIGURATION; Fucose; Fusobacterium necrophorum; growth & development; Hydrogen; Hydrolysis; immunology; isolation & purification; Laboratories; LINKAGES; LIPOPOLYSACCHARIDE; Liver Diseases; Magnetic Resonance Spectroscopy; mass spectrometry; Methods; Microbiology; Molecular Sequence Data; O-SPECIFIC POLYSACCHARIDE; Oligosaccharides; Polysaccharides,Bacterial; REPEATING UNIT; STRUCTURAL; TRISACCHARIDE
AbstractThe O-specific polysaccharide component of the lipopolysaccharide produced by Fusobacterium necrophorum is of the teichoic acid type, with repeating units connected by phosphoric diester linkages. Dephosphorylation of the polysaccharide by treatment with aqueous hydrogen fluoride yielded a carbohydrate composed of a trisaccharide linked to an acidic component. This product, and the polysaccharide, were investigated by chemical methods and 1H-, 13C-, 31P- and 15N-NMR spectroscopy and the former also by fast-atom-bombardment mass spectrometry. It is proposed that the polysaccharide is composed of repeating units having the following structure, in which Fuc represents fucose (6-deoxy-galactose), Am represents an acetamidino group and Sug 2,4-diamino-2,4,6-trideoxy-D-glucose ('bacillosamine') acetylated at the 2-position and acylated with a (S)-3-hydroxybutanoic acid at the 4-position. The acid was identified as a 2-amino-2-deoxy-2-C-methyl-pentonic acid (2-amino-2-methyl-3,4,5-trihydroxypentanoic acid). The configuration of this acid remains to be determined. [formula: see text]
Publication date
AffiliationNRC Institute for Biological Sciences; National Research Council Canada
Peer reviewedYes
NPARC number9377290
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Record identifierd04e2c0a-6e64-4958-a163-26940b9740d8
Record created2009-07-10
Record modified2016-06-01
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