Genomic analysis of carbon monoxide utilization and butanol production by clostridium carboxidivorans strain P7T

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DOIResolve DOI: http://doi.org/10.1371/journal.pone.0013033
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EditorSearch for: Ahmed, Niyaz
TypeArticle
Journal titlePLoS ONE
Volume5
Issue9 (e13033)
Pages112; # of pages: 12
AbstractIncreasing demand for the production of renewable fuels has recently generated a particular interest in microbial production of butanol. Anaerobic bacteria, such as Clostridium spp., can naturally convert carbohydrates into a variety of primary products, including alcohols like butanol. The genetics of microorganisms like Clostridium acetobutylicum have been well studied and their solvent-producing metabolic pathways characterized. In contrast, less is known about the genetics of Clostridium spp. capable of converting syngas or its individual components into solvents. In this study, the type of strain of a new solventogenic Clostridium species, C. carboxidivorans, was genetically characterized by genome sequencing. C. carboxidivorans strain P7T possessed a complete Wood-Ljungdahl pathway gene cluster, involving CO and CO2 fixation and conversion to acetyl-CoA. Moreover, with the exception of an acetone production pathway, all the genetic determinants of canonical ABE metabolic pathways for acetate, butyrate, ethanol and butanol production were present in the P7T chromosome. The functionality of these pathways was also confirmed by growth of P7T on CO and production of CO2 as well as volatile fatty acids (acetate and butyrate) and solvents (ethanol and butanol). P7T was also found to harbour a 19 Kbp plasmid, which did not include essential or butanol production related genes. This study has generated in depth knowledge of the P7T genome, which will be helpful in developing metabolic engineering strategies to improve C. carboxidivorans's natural capacity to produce potential biofuels from syngas.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); NRC Biotechnology Research Institute
Peer reviewedYes
NRC number53337
NPARC number16157238
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Record identifierd0ad0e42-c9dc-409a-95f8-58bf526f1421
Record created2010-11-05
Record modified2016-05-09
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