Monitoring of potential cytotoxic and inhibitory effects of titanium dioxide using on-line and non-invasive cell-based impedance spectroscopy

Alternative titleMonitoring of potential cytotoxic effects of titanium dioxide using on-line and non-invasive cell-based impedance spectroscopy
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DOIResolve DOI: http://doi.org/10.1016/j.aca.2013.03.044
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TypeArticle
Journal titleAnalytica Chimica Acta
ISSN00032670
Volume777
Pages7885; # of pages: 8
SubjectTitanium dioxide; cytotoxicity; inhibition; nanoparticles; nano materials; impedance biosensing
AbstractTitanium dioxide (TiO₂) nanoparticles (NPs) with different sizes and structures were probed for plausible cytotoxicity using electric cell-substrate impedance sensing (ECIS), a non-invasive and on-line procedure for continuous monitoring of cytotoxicity. For insect cells (Spodoptera frugiperda Sƒ9), the ECIS₅₀ values, i.e., the concentration required to achieve 50% inhibition of the response, differed depending on the size and shape of the TiO₂ nanostructure. The lowest ECIS₅₀ value (158 ppm) was observed for the needle shaped rutile TiO2 (10 nm × 40 nm, 15.5 nm nominal particle size), followed by 211 ppm for P-25 (34.1 nm, 80% anatase and 20% rutile), 302 ppm for MTI5 (5.9 nm, 99% anatase) and 417 ppm for Hombitan LW-S bulk TiO₂ (169.5 nm, 99% anatase). Exposure of TiO2 NPs to UV light at 254 nm or 365 nm exhibited no significant effect on the ECIS₅₀ value due to the aggregation of TiO₂ NPs with diminishing photocatalytic activities. Chinese hamster lung fibroblast V79 cells, exhibited no significant cytotoxicity/inhibition up to 400 ppm with P25, MTI₅ and bulk TiO₂. However, a noticeable inhibitory effect was observed (ECIS₅₀ value of 251 ppm) with rutile TiO₂ as cell spreading on the electrode surface was prevented.
Publication date
PublisherElsevier
LanguageEnglish
AffiliationNational Research Council Canada; Aquatic and Crop Resource Development; Human Health Therapeutics
Peer reviewedYes
NRC number55426
NPARC number21268411
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Record identifierd10f6f1d-7a8f-4964-8d40-2a6b246b4d8d
Record created2013-07-10
Record modified2016-05-09
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