Protein profiling in potato (Solanum tuberosum L.) leaf tissues by differential centrifugation

Download
  1. Get@NRC: Protein profiling in potato (Solanum tuberosum L.) leaf tissues by differential centrifugation (Opens in a new window)
DOIResolve DOI: http://doi.org/10.1021/pr201004k
AuthorSearch for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for:
TypeArticle
Journal titleJournal of Proteome Research
ISSN1535-3893
1535-3907
Volume11
Issue4
Pages25942601
Subjectpotato leaf; protein profiling; differential centrifugation; reproducibility; cell wall and cytoplasmic fractions
AbstractFoliar diseases, such as late blight, result in serious threats to potato production. As such, potato leaf tissue becomes an important substrate to study biological processes, such as plant defense responses to infection. Nonetheless, the potato leaf proteome remains poorly characterized. Here, we report protein profiling of potato leaf tissues using a modified differential centrifugation approach to separate the leaf tissues into cell wall and cytoplasmic fractions. This method helps to increase the number of identified proteins, including targeted putative cell wall proteins. The method allowed for the identification of 1484 nonredundant potato leaf proteins, of which 364 and 447 were reproducibly identified proteins in the cell wall and cytoplasmic fractions, respectively. Reproducibly identified proteins corresponded to over 70% of proteins identified in each replicate. A diverse range of proteins was identified based on their theoretical pI values, molecular masses, functional classification, and biological processes. Such a protein extraction method is effective for the establishment of a highly qualified proteome profile.
Publication date
LanguageEnglish
AffiliationNRC Institute for Marine Biosciences; National Research Council Canada
Peer reviewedYes
NPARC number21268910
Export citationExport as RIS
Report a correctionReport a correction
Record identifierd1841115-57a1-4b55-85ff-073d22268c15
Record created2013-11-25
Record modified2016-05-09
Bookmark and share
  • Share this page with Facebook (Opens in a new window)
  • Share this page with Twitter (Opens in a new window)
  • Share this page with Google+ (Opens in a new window)
  • Share this page with Delicious (Opens in a new window)