All-fiber multimodal CARS microscopy of live cells

DOIResolve DOI: http://doi.org/10.1109/CLEOE-EQEC.2009.5191479
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TypeArticle
Proceedings titleLasers and Electro-Optics 2009 and the European Quantum Electronics Conference. CLEO Europe - EQEC 2009. European Conference on
ConferenceLasers and Electro-Optics 2009 and the European Quantum Electronics Conference, June 14-19, 2009, Munich, Germany
ISBN978-1-4244-4080-1
978-1-4244-4079-5
Article number5191479
AbstractCoherent anti-Stokes Raman scattering (CARS) microscopy is a promising tool for live cell imaging and has been applied to the study of diseases such as multiple sclerosis, atherosclerosis and hepatitis. Combining PCFs with optimally chirped fs pulses, high performance multimodal (i.e. simultaneous two-photon fluorescence (TPF), second harmonic generation (SHG) and CARS) imaging of live cells and tissues is possible using only a single laser. Here the paper demonstrates a fully operational multimodal live cell and tissue imaging system based on a compact fs Er fiber oscillator system. A key component is a newly developed ultra-highly nonlinear silica fiber (UHNLF) which greatly reduces the peak power requirement for Stokes pulse generation. A commercial fully integrated frequency doubled fs Er fiber laser (IMRA F100) was the pump for TPF and SHG imaging. Here, in this paper a multimodal image of a fixed rabbit atherosclerotic arterial sample where TPF (elastin), SHG (collagen) and CARS (lipid) signals were collected simultaneously. This demonstrates for the first time the feasibility of a compact all-fiber source for multimodal CARS microscopy of live cells and tissue.
Publication date
LanguageEnglish
AffiliationNRC Steacie Institute for Molecular Sciences; National Research Council Canada
Peer reviewedNo
NPARC number16872068
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Record identifierd2d395fa-8a03-4ec7-b769-bb0ea5dca15c
Record created2011-02-15
Record modified2016-05-09
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