Pilot-scale supercritical carbon dioxide extractions for the recovery of triacylglycerols from microalgae : a practical tool for algal biofuels research

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DOIResolve DOI: http://doi.org/10.1007/s10811-011-9756-2
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TypeArticle
Journal titleJournal of Applied Phycology
Volume24
Issue3
Pages547555; # of pages: 9
AbstractWe describe the preliminary extractions from a pilot-scale supercritical carbon dioxide (SC-CO2) extractor for the isolation of algal lipids suitable for small-scale conversion to liquid hydrocarbon fuels. Flowable oils were recovered from SC-CO2 extractions of lyophilized Nannochloropsis granulata. The extracted oils were determined to be composed primarily of triacylglycerols (TAG) by liquid chromatography-mass spectrometry analysis. Gravimetric lipid yield was increased significantly from 15.56 to 28.45 mg g-1 ash-free dry weight (AFDW) with an increase in temperature from 50°C to 70°C, at 35 MPa over 270 min. Varying pressure had no significant effect on lipid yield. Liquid chromatography-mass spectrometry analysis of the SC-SO2 extracts indicated that the TAG profile remained constant regardless of extraction pressure, and analysis of fatty acid methyl esters (FAME) revealed a uniform profile throughout all extraction conditions. Our optimized gravimetric lipid yields from N. granulata (28.45 mg g-1 AFDW) were approximately half of the yields obtained by Soxhlet extraction with hexane (57.53 mg g-1 AFDW); however, the FAME yields were similar regardless of extraction technique (18.23 mg FAME g-1 and 17.35 mg FAME g-1 AFDW from SC-CO2 extraction and hexane extraction, respectively). Further extractions with Botryococcus braunii indicated that fatty acid extraction by SC-CO2 was as efficient as hexane extraction. These results highlight the suitability of SC-CO2 for large-scale oil extraction of microalgae for biofuel or biojet analyses due to its selectivity for TAG extraction.
Publication date
LanguageEnglish
AffiliationNRC Institute for Marine Biosciences; National Research Council Canada
Peer reviewedYes
NPARC number19649675
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Record identifierd450acec-031b-4b37-802a-c995772faae9
Record created2012-04-02
Record modified2016-05-09
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