Measurement of macrophage-mediated killing of intracellular bacteria, including Francisella and Mycobacteria

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DOIResolve DOI: http://doi.org/10.1002/0471142735.im1425s93
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TypeArticle
Journal titleCurrent Protocols in Immunology
ISBN9780471142737
IssueSuppl. 93
Article number14.25
Pages14.25.114.25.13
SubjectFrancisella; Mycobacteria; intracellular bacteria; macrophage; cytokine; intracellular killing; T lymphocytes
AbstractMacrophages activated by T cell cytokines are a critical defense mechanism against intracellular bacterial pathogens. This unit presents two general methods for assessing the capacity of mouse macrophages, activated with either soluble cytokines or whole immune T lymphocytes, to control or reduce numbers of intracellular bacteria residing within them. “Measurement of killing” is inferred from a reduction in the number of colony-forming units (cfu) of bacteria at the end of a culture period, compared to the input numbers of cfu at initiation of culture, to the peak numbers of cfu measured during culture, or to a control group in which killing is expected to be poor.
Publication date
LanguageEnglish
AffiliationNRC Institute for Biological Sciences; National Research Council Canada
Peer reviewedYes
NPARC number19230297
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Record identifierd49a80ec-f849-4114-a5eb-4fa4b59db687
Record created2012-03-08
Record modified2016-08-19
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