Direct electrochemistry of horseradish peroxidase immobilized on a monolayer modified nanowire array electrode

Download
  1. (PDF, 539 KB)
  2. Get@NRC: Direct electrochemistry of horseradish peroxidase immobilized on a monolayer modified nanowire array electrode (Opens in a new window)
DOIResolve DOI: http://doi.org/10.1016/j.bios.2009.10.018
AuthorSearch for: ; Search for: ; Search for: ; Search for: ; Search for:
TypeArticle
Journal titleBiosensors and Bioelectronics
Volume25
Issue6
Pages13131318; # of pages: 6
SubjectDirect electrochemistry; Horseradish peroxidase; Nanowire array electrode; Self-assembled monolayer
AbstractVertically aligned nanowire array electrodes (NAEs) were prepared by electrodeposition of gold into an anodic aluminium oxide membrane (AAM), providing an ordered three-dimensional (3D) matrix for immobilization of redox proteins. Third-generation H₂O₂ biosensors were prepared by covalent immobilization of horseradish peroxidase (HRP) on the self-assembled monolayer modified NAEs. Direct electron transfer and electrocatalytic performances of the HRP/NAEs with different nanowire lengths (deposition time of 2, 4 and 5 h) were investigated. Results showed that with longer nanowires, better performances were achieved. The HRP/NAE₅h (5 h deposition time) exhibited remarkable sensitivity (45.86μA mM−¹ cm−²) towards H₂O₂ with a detection limit of 0.42μM (S/N= 3), linearity up to 15mM and a response time of 4 s. The ordered 3D gold nanowire array with high conductivity, excellent electron transfer capability and good biocompatibility proved promising for fabricating sensitive, selective, stable and mediator-free enzymatic biosensors.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); NRC Biotechnology Research Institute
Peer reviewedYes
NRC number5004
NPARC number14148122
Export citationExport as RIS
Report a correctionReport a correction
Record identifierd587e41c-9148-4c2f-844c-fdf28c914e23
Record created2010-06-02
Record modified2016-05-09
Bookmark and share
  • Share this page with Facebook (Opens in a new window)
  • Share this page with Twitter (Opens in a new window)
  • Share this page with Google+ (Opens in a new window)
  • Share this page with Delicious (Opens in a new window)
Date modified: