Cytotoxic triterpenes from Antrodia camphorata and their mode of action in HT-29 human colon cancer cells

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DOIResolve DOI: http://doi.org/10.1016/j.canlet.2009.05.002
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TypeArticle
Journal titleCancer Letters
Volume285
Issue1
Pages7379; # of pages: 7
SubjectENV; antrodia camphorata; triterpenes; in vitro cytotoxicity; HT-29 cells; PARP
AbstractFive lanostane (2, 3, 4, 6 and 8) and three ergostane-type (1, 5 and 7) triterpenes isolated from the fruiting bodies of Antrodia camphorata were evaluated for their in vitro cytotoxic data against various cancer cell types. The three zhankuic acids, 1, 5 and 7 displayed the most potent cytotoxic effect with an IC50 value of 22.3–75.0 lM. The compound 3 was selectively cytotoxic in three colon cancer cell lines (HT-29, HCT-116 and SW-480) and a breast cancer model (MDA-MB-231), whereas 8 only showed its cytotoxicity against MDA-MB-231. None of these isolates was toxic to mammary epithelial (MCF10A) and primary foreskin fibroblast (HS68) cells, two human normal cell lines. The compounds 1, 5 and 7 were also demonstrated to induce apoptosis in HT-29 and SW-480 cells, as confirmed by sub-G1 cell cycle arrest. In HT-29 cells, the expression of apoptosis-associated proteins poly-(ADP-ribose) polymerase cleavage, Bcl-2 and procaspase-3 were suppressed by compounds 1, 5 and 7. A mixture containing 4 µM each of compounds 1, 5 and 7 also showed a synergistic cytotoxic effect in HT-29 cells.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada; NRC Biotechnology Research Institute
Peer reviewedNo
NRC number49998
NPARC number12723983
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Record identifierd59d2114-43e0-4dc6-b632-9ddf3d02162f
Record created2009-10-26
Record modified2016-05-09
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