Hyperspectral multimodal CARS microscopy in the fingerprint region

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DOIResolve DOI: http://doi.org/10.1002/jbio.201200171
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TypeArticle
Journal titleJournal of Biophotonics
ISSN1864-063X
Volume7
Issue1-Feb
Pages4958; # of pages: 10
AbstractA simple scheme for multimodal coherent anti-Stokes Raman scattering (CARS) microscopy is based on the spectral focusing of ultrafast-oscillator-derived pump/probe light and synchronous photonic crystal fiber (PCF) fiber-generated broadband Stokes light. To date, such schemes allowed rapid hyperspectral imaging throughout the CH/OH high frequency region (2700-4000 cm-1). Here we extend this approach to the middle (1640-3300 cm-1) and fingerprint regions (850-1800 cm-1) of the Raman spectrum. Our simple integrated approach to rapid hyperspectral CARS microscopy in the fingerprint region is demonstrated by applications to label-free multimodal imaging of cellulose and bulk bone, including use of the phosphate resonance at 960 cm-1. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC)
Peer reviewedYes
NPARC number21270809
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Record identifierd6659aac-4f50-42eb-9490-3a215a53a2a5
Record created2014-02-17
Record modified2016-05-09
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