Functional characterization of the initiation enzyme of S-layer glycoprotein glycan biosynthesis in Geobacillus stearothermophilus NRS 2004/3a: J.Bacteriol.

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TypeArticle
Journal titleJ Bacteriol.
Volume189
Issue7
Pages25902598; # of pages: 9
SubjectAmino Acid Sequence; Bacillaceae; Bacteria; bacterial; Bacterial Proteins; biosynthesis; Carbohydrate Sequence; CHAIN; chemistry; CLUSTER; Conserved Sequence; ENZYME; Escherichia; Escherichia coli; ESCHERICHIA-COLI; Galactosyltransferases; GENE; genetics; GLYCAN CHAIN; glycoprotein; Glycoproteins; Glycosylation; In Vitro; Linker; Lipopolysaccharides; membrane; Membrane Glycoproteins; metabolism; Molecular Sequence Data; Plasmids; POLYSACCHARIDE; Polysaccharides; protein; Protein Conformation; REPEATING UNIT; RESIDUES; Salmonella; Sequence Alignment; Sequence Homology,Amino Acid; STRAIN; STRAINS; surface; transfer; transferase; TRISACCHARIDE; UNIT
AbstractThe glycan chain of the S-layer glycoprotein of Geobacillus stearothermophilus NRS 2004/3a is composed of repeating units [-->2)-alpha-l-Rhap-(1-->3)-beta-l-Rhap-(1-->2)-alpha-l-Rhap-(1-->], with a 2-O-methyl modification of the terminal trisaccharide at the nonreducing end of the glycan chain, a core saccharide composed of two or three alpha-l-rhamnose residues, and a beta-d-galactose residue as a linker to the S-layer protein. In this study, we report the biochemical characterization of WsaP of the S-layer glycosylation gene cluster as a UDP-Gal:phosphoryl-polyprenol Gal-1-phosphate transferase that primes the S-layer glycoprotein glycan biosynthesis of Geobacillus stearothermophilus NRS 2004/3a. Our results demonstrate that the enzyme transfers in vitro a galactose-1-phosphate from UDP-galactose to endogenous phosphoryl-polyprenol and that the C-terminal half of WsaP carries the galactosyltransferase function, as already observed for the UDP-Gal:phosphoryl-polyprenol Gal-1-phosphate transferase WbaP from Salmonella enterica. To confirm the function of the enzyme, we show that WsaP is capable of reconstituting polysaccharide biosynthesis in WbaP-deficient strains of Escherichia coli and Salmonella enterica serovar Typhimurium
Publication date
LanguageEnglish
AffiliationNRC Institute for Biological Sciences; National Research Council Canada
Peer reviewedNo
NRC numberSTEINER2007
NPARC number9364325
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Record identifierd6804f6f-3573-4485-b800-2872911318b9
Record created2009-07-10
Record modified2016-05-09
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