Structural and immunochemical characterization of the type VIII group B Streptococcus capsular polysaccharide: J.Biol.Chem.

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TypeArticle
Journal titleJournal Of Biological Chemistry
Volume271
Issue15
Pages87868790; # of pages: 5
Subject13C; 13C-NMR; 1H; 1H-NMR; ACID; Acids; ANTIGEN; Antigens; Antigens,Bacterial; bacterial; Bacterial Capsules; Canada; capsular polysaccharide; capsule; Carbohydrate Sequence; chemistry; D-GLUCOSE; group B Streptococcus; GROUP-B; HIGH-RESOLUTION; IMMUNOCHEMICAL CHARACTERIZATION; immunology; MAGNETIC; Magnetic Resonance Spectroscopy; MAGNETIC-RESONANCE; Meningitis; Molar; Molecular Sequence Data; Neuraminidase; NMR; NUCLEAR; Nuclear Magnetic Resonance; NUCLEAR-MAGNETIC-RESONANCE; Oxidation-Reduction; POLYSACCHARIDE; REPEATING UNIT; RESOLUTION; RESONANCE; SIALIC; Sialic Acids; SIALIC-ACID; STRAIN; Streptococcus; Streptococcus agalactiae; STRUCTURAL; structure; Support,U.S.Gov't,P.H.S.; UNIT
AbstractThe type VIII capsular polysaccharide has been isolated and purified from a newly described strain of group B Streptococcus which is a leading cause of sepsis and neonatal meningitis in Japan. The polysaccharide contains D-glucose, D-galactose, L-rhamnose, and sialic acid in the molar ratio 1:1:1:1. By means of high resolution 1H nuclear magnetic resonance (1H NMR), 13C NMR, and homo- and heterocorrelated NMR, the repeating unit structure of the type VIII polysaccharide was delineated as the following, [formula: see text] Enzymatic studies established this polysaccharide as the first from which sialic acid, linked to a branched beta-D-galactopyranosyl residue, is known to be removed by bacterial neuraminidase
Publication date
LanguageEnglish
AffiliationNRC Institute for Biological Sciences; National Research Council Canada
Peer reviewedNo
NRC numberKOGAN1996
NPARC number9364359
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Record identifierd70dd315-1daf-4e1a-ae56-d58768d6b8ba
Record created2009-07-10
Record modified2016-05-09
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