The chemical constitution of lipid A from Serratia marcescens

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DOIResolve DOI: http://doi.org/10.1139/o70-010
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TypeArticle
Journal titleCanadian Journal of Biochemistry
ISSN0008-4018
Volume48
Issue1
Pages5562; # of pages: 8
AbstractThe lipopolysaccharide of Serratia marcescens, under mild hydrolysis conditions (0.25 N H2SO4 for 2.5 h at 100°), yielded a lipid A containing D-glucosamine, fatty acids, acetyl and phosphate groups (approximate molar ratio 3:9:4:2), a small amount of ethanolamine, and a trace of galactosamine. Lipid A on methylation and subsequent hydrolysis yielded 3,4-di-O-methyl-D-glucosamine and 3,4,6-tri-O-methyl-D-glucosamine identified by gas–liquid chromatography as their glucitol acetates. These sugars were further identified by ninhydrin degradation to yield 2,3-di-O-methyl-L-arabinose and 2,3,5-tri-O-methyl-L-arabinose. From these findings, it was concluded that the D-glucosamine units in the lipid A were linked 1 → 6. A negative optical rotation of the lipid A suggested a β linkage. On the basis of the glycosidic linkage evidence and additional compositional data, a possible structure for the lipid A of S. marcescens is proposed and discussed in detail.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada
Peer reviewedYes
NRC number11012
NPARC number21274542
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Record identifierd73aed0e-fb46-4bd1-94c7-1fbc7ceeb3a5
Record created2015-03-16
Record modified2016-05-09
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