Study of matrix additives for sensitive analysis of lipid A by matrix-assisted laser desorption ionization mass spectrometry

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DOIResolve DOI: http://doi.org/10.1128/AEM.03082-09
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TypeArticle
Journal titleApplied and Environmental Microbiology
ISSN0099-2240
1098-5336
Volume76
Issue11
Pages34373443; # of pages: 7
Subjectlipopolysaccharide; endotoxin; lipid A; phosphorylation; MALDI-TOF
AbstractMatrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) has been widely used for structural characterization of bacterial endotoxins (lipid A). However, the mass spectrometric behavior of the lipid A molecule is highly dependent on the matrix. Furthermore, this dependence is strongly linked to phosphorylation patterns. Using lipid A from Escherichia coli O116 as a model system, we have investigated the effects of different matrices and comatrix compounds on the analysis of lipid A. In this paper, we report a highly sensitive matrix system for lipid A analysis, which consists of 5-chloro-2-mercaptobenzothiazole matrix and EDTA ammonium salt comatrix. This matrix system enhances the sensitivity of the analysis of diphosphorylated lipid A species by more than 100-fold and in addition provides tolerance to high concentrations of sodium dodecyl sulfate (SDS) and tolerance to sodium chloride and calcium chloride at 10 μM, 100 μM, and 10 μM concentrations. The method was further evaluated for analysis of lipid A species with different phosphorylation patterns and from different bacteria, including Helicobacter pylori, Salmonella enterica serovar Riogrande, and Francisella novicida.
Publication date
PublisherAmerican Society for Microbiology
LanguageEnglish
AffiliationNRC Institute for Biological Sciences; National Research Council Canada
Peer reviewedYes
NPARC number17400936
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Record identifierd78d1c35-bebe-4622-a7a3-2563d10f55de
Record created2011-03-25
Record modified2016-08-19
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