Multilabeling biomolecules at a single site. 1. Synthesis and characterization of a dendritic label for electrochemiluminescence assays

Download
  1. (PDF, 485 KB)
  2. Get@NRC: Multilabeling biomolecules at a single site. 1. Synthesis and characterization of a dendritic label for electrochemiluminescence assays (Opens in a new window)
DOIResolve DOI: http://doi.org/10.1021/ac034664d
AuthorSearch for: ; Search for: ; Search for: ; Search for:
TypeArticle
Journal titleAnalytical Chemistry
Volume75
Issue23
Pages67086717; # of pages: 10
AbstractUltrasensitive bioanalytical assays are of great value for early detection of human diseases and pathogens. The sensitivities of immunoassays and DNA probing can be enhanced by multilabeling the biorecognition partner used for affinity-based assays. However, the bioreactivity of biomolecules is affected by a high degree of multilabeling at multiple functional sites. It is proposed that dendritic scaffoldings be used to link multiple signal-generating units to a single site with potentially minimum impact on the bioaffinity. A prototype label, a zeroth-generation dendron, bearing three [Ru(bpy)₃]²⁺ units for electrochemiluminescence (ECL) assays was synthesized and characterized preliminarily by spectroscopic, electrochemical, and ECL methods. No evidence of interaction between the neighboring [Ru(bpy)₃]²⁺ units in the label molecule was found from these characterizations. Both the photoluminescence and ECL of the prototype label have features very similar to those of mononuclear [Ru- (bpy)₃]²⁺ compounds. Labeling a model protein with a triad of [Ru(bpy)₃]²⁺ at one NH₂ position was demonstrated. The results reported here provide support to applying the proposed multilabeling strategy to affinitybased bioanalytical assays.
Publication date
LanguageEnglish
AffiliationNRC Institute for Chemical Process and Environmental Technology; National Research Council Canada; NRC Institute for National Measurement Standards
Peer reviewedYes
NRC number51678
NPARC number14542472
Export citationExport as RIS
Report a correctionReport a correction
Record identifierd795fddf-c5ee-4f0a-a89f-f0f12ff122f8
Record created2010-03-01
Record modified2016-05-09
Bookmark and share
  • Share this page with Facebook (Opens in a new window)
  • Share this page with Twitter (Opens in a new window)
  • Share this page with Google+ (Opens in a new window)
  • Share this page with Delicious (Opens in a new window)