A structural genomics pilot project based on gene targets selected from Escherichia coli

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DOIResolve DOI: http://doi.org/10.1016/S0022-0248(01)01155-1
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TypeArticle
Journal titleJournal of Crystal Growth
Volume232
Issue1-4
Pages421425; # of pages: 5
Subjectgenome; pha; A1. Biocrystallization; A1. Crystal structure; A1. Purification; A1. X-ray diffraction
AbstractA pilot project based on gene targets selected from the genome of E. coli has been initiated with 38 genes for initial cloning. Of these, 18 proteins have been purified to date and some crystals were obtained for twelve of them. Of these, four proteins yielded crystals diffracting to a sufficiently high resolution to warrant structural investigation. We have determined 3-D structures of three of these proteins using Se-Met labeling and MAD methods, while the structure of the fourth one was simultaneously determined by another group. To manage the parallel work on many proteins by several researchers it became necessary to create a searchable database containing the pertinent information about every stage of the work.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada; NRC Biotechnology Research Institute
Peer reviewedNo
NRC number44826
NPARC number3539584
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Record identifierd82392fd-859b-41c8-9c67-eb715fc5862f
Record created2009-03-01
Record modified2016-05-09
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