In vivo monitoring of the distribution of a monoclonal antibody fragment

DOIResolve DOI:
AuthorSearch for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for:
Proceedings titleVibrational Spectroscopy
ConferenceShedding New Light on Disease : Optical Diagnostics for the New Millennium, June 25-30, 2000, Winnipeg, Canada
Pages177188; # of pages: 12
SubjectImaging; Monoclonal antibody; Nude mouse; Non-invasive; Cy5.5
AbstractThis study demonstrates the potential of in vivo, whole body fluorescence imaging for pharmacokinetic studies. The distribution of a novel human anti-tumour antibody fragment, NovoMab-G2-scFv, labelled with a fluorescent dye (Cy5.5.18) was monitored in vivo. The NovoMab-G2-scFv–Cy5 complex was injected via the tail vein into nude mice bearing subcutaneous human melanoma tumour cells. The distribution of fluorescence NovoMab-G2-scFv–Cy5 was imaged non-invasively using a charge-coupled device (CCD) camera. Whole body fluorescence images were acquired 2, 6, 12, 24, 48 and 72 h post-injection. Fluorescence was detected at the tumour site following injection of NovoMab-G2-scFv–Cy5 but not following injection of a labelled irrelevant antibody fragment, demonstrating specific binding of the antibody–dye complex to the tumour. Fluorescence from the tumour site peaked 2 h post-injection and gradually declined, reaching a minimum 72 h post-injection. Fitting an exponential decay to fluorescence data extracted from images allowed the half-time of the antibody at the tumour site to be calculated, and a value of 7.7 h was obtained. Fluorescence was also apparent in the kidneys, indicating clearance of the NovoMab-G2-scFv through the kidneys. Again, fluorescence intensity decreased with time, reaching a minimum 72 h post-injection. Imaging of isolated organs (ex vivo) confirmed the presence of the antibody–dye complex in the tumours, kidneys and liver. No fluorescence was observed in the brain, heart, lungs or spleen, suggesting that these organs do not accumulate the NovoMab-G2-scFv–dye complex.
Publication date
PublisherElsevier B.V.
AffiliationNRC Institute for Biodiagnostics; National Research Council Canada
Peer reviewedYes
NRC number1882
NPARC number9148119
Export citationExport as RIS
Report a correctionReport a correction
Record identifierd8672d57-a496-435a-bb7c-0729970c27c7
Record created2009-06-25
Record modified2016-09-23
Bookmark and share
  • Share this page with Facebook (Opens in a new window)
  • Share this page with Twitter (Opens in a new window)
  • Share this page with Google+ (Opens in a new window)
  • Share this page with Delicious (Opens in a new window)