Use of a bacterial antimicrobial resistance gene microarray for the identification of resistant Staphylococcus aureus

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DOIResolve DOI: http://doi.org/10.1111/j.1863-2378.2010.01358.x
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TypeArticle
Journal titleZoonoses and Public Health
Volume57
IssueSuppl. 1
Pages9499; # of pages: 6
SubjectMSSA; Staphylococcus aureus; antimicrobial resistance; microarray; activity; analysis; Animal; Animals; antibiotic resistance; antibiotic sensitivity; Antimicrobial resistance; article; Bacteria; bio; Biotechnology; blood; Canada; Cattle; cotrimoxazole; crossector; Dna; DNA microarray; Enterococcus faecium; env; epidemiology; erythromycin; Escherichia coli; Evolution; Gene transfer; Genes; genetic screening; Genomics; genotype; Human; Humans; Hybridization; I; In Vitro; lincomycin; Macrolides; methods; Microarray; Microarrays; minimum inhibitory concentration; MSSA; nonhuman; novobiocin; NPArC; oxacillin; PCR; penicillin G; Phenotype; priority journal; Staphylococcus aureus; tetracycline; Virulence; Vitro
AbstractAs diagnostic and surveillance activities are vital to determine measures needed to control antimicrobial resistance (AMR), new and rapid laboratory methods are necessary to facilitate this important effort. DNA microarray technology allows the detection of a large number of genes in a single reaction. This technology is simple, specific and high-throughput. We have developed a bacterial antimicrobial resistance gene DNA microarray that will allow rapid antimicrobial resistance gene screening for all Gram-positive and Gram-negative bacteria. A prototype microarray was designed using a 70-mer based oligonucleotide set targeting AMR genes of Gram-negative and Gram-positive bacteria. In the present version, the microarray consists of 182 oligonucleotides corresponding to 166 different acquired AMR gene targets, covering most of the resistance genes found in both Gram-negative and -positive bacteria. A test study was performed on a collection of Staphylococcus aureus isolates from milk samples from dairy farms in Qu�bec, Canada. The reproducibility of the hybridizations was determined, and the microarray results were compared with those obtained by phenotypic resistance tests (either MIC or Kirby-Bauer). The microarray genotyping demonstrated a correlation between penicillin, tetracycline and erythromycin resistance phenotypes with the corresponding acquired resistance genes. The hybridizations showed that the 38 antimicrobial resistant S. aureus isolates possessed at least one AMR gene.
Publication date
LanguageEnglish
AffiliationNRC Biotechnology Research Institute; National Research Council Canada
Peer reviewedYes
NRC number52774
NPARC number16616689
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Record identifierd89acf9c-88c9-402f-93c9-b5639f51f277
Record created2011-03-31
Record modified2016-05-09
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