Biomolecular sample considerations essential for optimal performance from cryogenic probes

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DOIResolve DOI: http://doi.org/10.1007/s11306-013-0620-z
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TypeArticle
Journal titleMetabolomics
ISSN1573-3882
Pages607615; # of pages: 9
Subject1.7 and 5 mm cryogenic probes; Biomolecular samples; Metabolomic data collection; Quantitation; Salt tolerance
AbstractFor compounds dissolved in non-polar solvents, nuclear magnetic resonance spectroscopic investigations have benefited greatly from the advent of cryogenically cooled probes. Unfortunately the allure of significant increases in sensitivity may not be realized for compounds such as metabolites that are dissolved in solvents with high ionic-strengths such as solutions typically utilized for metabolomic or biomolecular investigations. In some cases there is little benefit from a cryogenically cooled probe over a conventional room temperature probe. Various sample preparation methods have been developed to minimize the detrimental effects of salt; for large numbers of metabolomic samples these preparation methods tend to be onerous and impractical. An alternative to manipulating the sample, is to utilize a probe that is designed to have a higher tolerance for solutions with high ionic-strengths. In order to acquire high-quality optimal data and choose the appropriate probe configuration (especially important for comparative quantitative investigations) the effects of salts and buffers on cryogenic probe performance must be understood. Herein we detail sample considerations for two cryogenic probes, a standard 5 mm and a narrow diameter 1.7 mm, in an effort to identify via integrals, intensities and noise levels the optimal choice for biomolecular investigations.
Publication date
LanguageEnglish
AffiliationAquatic and Crop Resource Development; National Research Council Canada
Peer reviewedYes
NRC numberNRC-ACRD-55947
NPARC number21270824
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Record identifierd941bc73-8510-497c-a809-1d38751cb110
Record created2014-02-17
Record modified2016-05-09
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