A simple mathematical model for electric cell-substrate impedance sensing with extended applications

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DOIResolve DOI: http://doi.org/10.1016/j.bios.2009.12.025
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TypeArticle
Journal titleBiosensors and Bioelectronics
Volume25
Issue7
Pages17741780; # of pages: 7
SubjectElectric cell-substrate impedance sensing; Mathematical model; L-Cysteine; Ferri/ferrocyanide; Bacteria; Doubling time; Antibiotics resistance
AbstractThis paper presents a simple mathematical model to predict the impedance data acquired by electric cell-substrate impedance sensing (ECIS) at frequencies between 25 Hz and 60 kHz. With this model, the equivalent resistance (R) and capacitance (C) of biological samples adhered on gold surfaces could be more precisely measured at 4 kHz. ECIS applications were extended for real-time monitoring of living bacteria cultivated in Luria Bertani (LB) culture medium by two different approaches. In the former, we used a ferri/ferrocyanide redox couple in LB medium as an indicator for bacterial multiplication. Because the redox couple was toxic to some bacteria, we developed a second approach, in which L-cysteine self-assembled monolayers (SAM) on gold electrodes were used to detect living bacteria. The L-cysteine SAM could also be detected by ECIS. Unlike traditional impedance microbiological methods which need special culture media with low ions, our procedures significantly enhanced signal/noise ratios so bacteria could be detected in general purpose culture media. It was easy and convenient to obtain bacterial doubling times and evaluate the resistance of bacteria to antibiotics from ECIS spectra.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); NRC Biotechnology Research Institute
Peer reviewedYes
NRC number50007
NPARC number14758187
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Record identifierd9eb686d-9e9e-463e-95df-bd1cd9561b1b
Record created2010-06-02
Record modified2016-05-09
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