Detection of fluorine labeled Herceptin using cellular 19F MRI ex vivo

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DOIResolve DOI: http://doi.org/10.1016/j.jpba.2009.10.008
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TypeArticle
Journal titleJournal of Pharmaceutical and Biomedical Analysis
Volume51
Issue4
Pages894900; # of pages: 7
SubjectDrug efficacy; 19F MRI; Breast cancer cells
AbstractThe aim of this study was to assess the Herceptin efficacy in ex vivo cultures of MCF-7 breast carcinoma cells. Herceptin was used with perfluorooctyl bromide (PFOB) and conjugated with Lipoplex, containing plasmid DNA and Lipofectamine (LipA), to allow fluorine-19 magnetic resonance imaging (19F MRI) study. Treatments such as Herceptin, Herceptin/PFOB and Herceptin/PFOB/Lipoplex were used for ex vivo targeting of MCF-7 cells cultured in three-dimensional (3D) geometry using hollow fiber bioreactor (HFB) device. The viability of MCF-7 cells after 72 h treatments decreased to 54 ± 2%, 50 ± 3% and 45 ± 1% for Herceptin, Herceptin/PFOB and Herceptin/PFOB/Lipoplex, respectively. The EC50 values were 1000 μg/ml, 930 μg/ml and 730 μg/ml, respectively. The significant correlation between the treatment concentration and efficacy was observed in MCF-7 cell cultures.
Publication date
LanguageEnglish
AffiliationNRC Institute for Biodiagnostics; National Research Council Canada
Peer reviewedYes
NPARC number17653082
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Record identifierd9f1b38e-4089-47e9-ae6c-8cb881f596b1
Record created2011-04-03
Record modified2016-05-09
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