Overexpression of a novel biotrophy-specific colletotrichum truncatum Effector, CtNUDIX, in hemibiotrophic fungal phytopathogens causes incompatibility with their host plants

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DOIResolve DOI: http://doi.org/10.1128/EC.00192-12
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TypeArticle
Journal titleEukaryotic Cell
ISSN1535-9778
1535-9786
Volume12
Issue1
Pages211; # of pages: 10
AbstractThe hemibiotrophic fungus Colletotrichum truncatum causes anthracnose disease on lentils and a few other grain legumes. It shows initial symptomless intracellular growth, where colonized host cells remain viable (biotrophy), and then switches to necrotrophic growth, killing the colonized host plant tissues. Here, we report a novel effector gene, CtNUDIX, from C. truncatum that is exclusively expressed during the late biotrophic phase (before the switch to necrotrophy) and elicits a hypersensitive response (HR)-like cell death in tobacco leaves transiently expressing the effector. CtNUDIX homologs, which contain a signal peptide and a Nudix hydrolase domain, may be unique to hemibiotrophic fungal and fungus-like plant pathogens. CtNUDIX lacking a signal peptide or a Nudix motif failed to induce cell death in tobacco. Expression of CtNUDIX:eGFP in tobacco suggested that the fusion protein might act on the host cell plasma membrane. Overexpression of CtNUDIX in C. truncatum and the rice blast pathogen, Magnaporthe oryzae, resulted in incompatibility with the hosts lentil and barley, respectively, by causing an HR-like response in infected host cells associated with the biotrophic invasive hyphae. These results suggest that C. truncatum and possibly M. oryzae elicit cell death to signal the transition from biotrophy to necrotrophy.
Publication date
PublisherAmerican Society of Microbiology
LanguageEnglish
AffiliationNational Research Council Canada; Aquatic and Crop Resource Development
Peer reviewedNo
NRC number55435
NPARC number21268508
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Record identifierda1a6c6a-0e81-424a-ad46-f4493850c816
Record created2013-09-03
Record modified2016-05-09
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