Spontaneously formed monodisperse biomimetic unilamellar vesicles : the effect of charge, dilution, and time

Download
  1. Get@NRC: Spontaneously formed monodisperse biomimetic unilamellar vesicles : the effect of charge, dilution, and time (Opens in a new window)
DOIResolve DOI: http://doi.org/10.1016/S0006-3495(04)74316-7
AuthorSearch for: ; Search for: ; Search for: ; Search for: ; Search for:
TypeArticle
Journal titleBiophysical Journal
Volume86
Issue4
Pages26152629; # of pages: 15
AbstractUsing small-angle neutron scattering and dynamic light scattering, we have constructed partial structural phase diagrams of lipid mixtures composed of the phosphatidylcholines dimyristoyl and dihexanoyl doped with calcium ions (Ca2+) and/or the negatively charged lipid, dimyristoyl phosphatidylglycerol (DMPG). For dilute solutions (lipid concentration [<=]1 wt %), spontaneously forming unilamellar vesicles (ULVs) were found, and their polydispersity was determined to be [~]20%. The stability of the Ca2+- or DMPG-doped ULVs was monitored over a period of 4 days and their structural parameters (e.g., average outer radius, [<]Ro[>]) were found to be insensitive to the lipid concentration (Clp). However, doping the dimyristoyl/dihexanoyl system with both Ca2+ and DMPG resulted in ULVs whose [<]Ro[>] was found to be Clp dependent. The [<]Ro[>] of DMPG-doped ULVs remained unchanged over an extended period of time (at least 4 days), a good indication of their stability.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada; NRC Steacie Institute for Molecular Sciences
Peer reviewedNo
NPARC number12328651
Export citationExport as RIS
Report a correctionReport a correction
Record identifierdb1fdabe-1d03-43a4-bc21-586082e17301
Record created2009-09-10
Record modified2016-05-09
Bookmark and share
  • Share this page with Facebook (Opens in a new window)
  • Share this page with Twitter (Opens in a new window)
  • Share this page with Google+ (Opens in a new window)
  • Share this page with Delicious (Opens in a new window)