Growth and positioning of adipose-derived stem cells in microfluidic devices

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DOIResolve DOI: http://doi.org/10.1039/c2lc40891k
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TypeArticle
Journal titleLab on a Chip
ISSN1473-0197
1473-0189
Volume12
Issue22
Pages48294834
AbstractStem cells hold great promise for treatment of various degenerative diseases. However, clinical studies have only shown very moderate benefits of cell therapy. We believe that insufficiency of therapeutic benefits is due to limited homing of implanted stem cells to targeted organs. Microfluidic devices are a very useful research tool for quantitative characterizations of stem cells. The present study therefore was to assess the effects of epidermal growth factor (EGF) and direct current electric field (dcEF) on the growth and trafficking of adipose-derived stem cells (ASC). It was found that EGF did not affect cell proliferation in cell-culture flasks. However, ASC proliferated at a higher rate in microfluidic devices with continuous infusion of EGF. Furthermore, we found that ASC migrated toward an EGF gradient in microfluidic devices. Moreover, we found that ASC tended to position perpendicularly to dcEF. The results suggest that EGF and dcEF may be effective in guiding homing and trafficking of implanted ASC.
Publication date
LanguageEnglish
AffiliationMedical Devices; National Research Council Canada
Peer reviewedYes
NPARC number21268954
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Record identifierdb97ca83-7acd-441a-bec9-b871c5fdd813
Record created2013-11-27
Record modified2016-05-09
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