Design and dynamic culture of 3D-scaffolds for cartilage tissue engineering

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Journal titleJournal of Biomaterials Applications
Pages429444; # of pages: 16
SubjectCartilage repair; Cartilage tissue engineering; Cell attachments; Cell viability; Chondrocytes; Compressive strain; Culture conditions; Different pore sizes; Dynamic cell cultures; Dynamic loadings; Healthy tissues; In-cell; In-vitro; Interconnected pore networks; Loading condition; Macroporous; Poly-L-lactide; Porogen leaching; Porogens; Scaffold properties; solid freeform fabrication; Static and dynamic loading; Static conditions; Body fluids; Bromine compounds; Cartilage; Cell culture; Dynamic loads; Growth kinetics; Leaching; Loading; Microporosity; Optimization; Organic polymers; Pore size; Scanning electron microscopy; Three dimensional; Tissue engineering; Scaffolds; biomaterial; biomimetic material; tissue scaffold; animal; article; articular cartilage; biomechanics; cartilage cell; cattle; cell adhesion; cell survival; cytology; dog; in vitro study; male; materials testing; methodology; physiology; porosity; scanning electron microscopy; tissue engineering; Animals; Biocompatible Materials; Biomechanics; Biomimetic Materials; Cartilage, Articular; Cattle; Cell Adhesion; Cell Survival; Chondrocytes; Dogs; Male; Materials Testing; Microscopy, Electron, Scanning; Porosity; Tissue Engineering; Tissue Scaffolds
AbstractEngineered scaffolds for tissue-engineering should be designed to match the stiffness and strength of healthy tissues while maintaining an interconnected pore network and a reasonable porosity. In this work, we have used 3D-plotting technique to produce poly-L-Lactide macroporous scaffolds with two different pore sizes. The ability of these macroporous scaffolds to support chondrocyte attachment and viability were compared under static and dynamic loading in vitro. Moreover, the 3D-plotting technique was combined with porogen-leaching, leading to macro/microporous scaffolds, so as to examine the effect of microporosity on the level of cell attachment and viability under similar loading condition. Canine chondrocytes' cells were seeded onto the scaffolds with different topologies, and the constructs were cultured for up to 2 weeks under static conditions or in a bioreactor under dynamic compressive strain of 10% strain, at a frequency of 1 Hz. The attachment and cell growth of chondrocytes were examined by scanning electron microscopy and by 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. A significant difference in cell attachment was observed in macroporous scaffolds with different pore sizes after 1, 7, and 14 days. Cell viability in the scaffolds was enhanced with decreasing pore size and increasing microporosity level throughout the culture period. Chondrocyte viability in the scaffolds cultured under dynamic loading was significantly higher (p<0.05) than the scaffolds cultured statically. Dynamic cell culture of the scaffolds improved cell viability and decreased the time of in vitro culture when compared to statically cultured constructs. Optimizing the culture conditions and scaffold properties could generate optimal tissue/constructs combination for cartilage repair. © 2011 The Author(s).
Publication date
AffiliationNational Research Council Canada (NRC-CNRC); NRC Industrial Materials Institute (IMI-IMI)
Peer reviewedYes
NPARC number21271591
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Record identifierdbc2532b-794a-434b-9809-a177e54bb43f
Record created2014-03-24
Record modified2016-05-09
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