A new subfamily of short bacterial adenylate kinases with the Mycobacteriumtuberculosis enzyme as a model : a predictive and experimental study

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DOIResolve DOI: http://doi.org/10.1002/(SICI)1097-0134(19990801)36:2<238::AID-PROT9>3.0.CO;2-K
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TypeArticle
Journal titleProteins: Structure, Function, and Bioinformatics
ISSN1097-0134
Volume36
Issue2
Pages238248
Subjectadenylate kinase; short variant; Mycobacterium tuberculosis; NMR spectroscopy; infrared spectroscopy
AbstractThe adk gene from Mycobacterium tuberculosis codes for an enzyme of 181 amino acids. A sequence comparison with 52 different forms of adenylate kinases (AK) suggests that the enzyme from M. tuberculosis belongs to a new subfamily of “short” bacterial AKs. The recombinant protein, overexpressed in Escherichia coli, exhibits a low catalytic activity and an unexpectedly high thermal stability (Tm = 64.8°C). Based on various spectroscopic data, on the known three-dimensional structure of the AK from E. coli and on secondary structure predictions for various sequenced AKs, we propose a structural model for AK from M. tuberculosis (AKmt).
Publication date
PublisherWiley-Liss, Inc.
LanguageEnglish
AffiliationNRC Institute for Biodiagnostics; National Research Council Canada
Peer reviewedYes
NPARC number23000809
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Record identifierdc2407be-ee1f-4967-95bc-9e7968f26887
Record created2016-10-12
Record modified2016-10-12
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