Picomolar detection of protease using peptide/single walled carbon nanotube/gold nanoparticle-modified electrode

Download
  1. (PDF, 1 MB)
  2. Get@NRC: Picomolar detection of protease using peptide/single walled carbon nanotube/gold nanoparticle-modified electrode (Opens in a new window)
DOIResolve DOI: http://doi.org/10.1021/nn8000774
AuthorSearch for: ; Search for: ; Search for:
TypeArticle
Journal titleACS Nano
Volume2
Issue5
Pages10511057; # of pages: 7
SubjectENV; protease; ferrocene-pepstatin inhibitor; carbon nanotubes; gold nanoparticles; biosensor
AbstractPicomolar electrochemical detection of human immunodeficiency virus type-1 protease (HIV-1 PR) using ferrocene (Fc)-pepstatin-modified surfaces has been presented. Gold electrode surface was modified with gold nanoparticles (AuNP) or thiolated single walled carbon nanotubes/gold nanoparticles (SWCNT/AuNP). Thiol-terminated Fc-pepstatin was then self-assembled on such surfaces as confirmed by Raman spectroscopy and scanning electron microscope. The interaction between the Fc-pepstatin-modified substrates and HIV-1 PR was studied by cyclic voltammetry and electrochemical impedance spectroscopy. Both electrode materials showed enhanced electrochemical responses to increasing concentrations of HIV-1 PR with shifting to higher potentials as well as decrease in the overall signal intensity. However, the sensing electrode modified with thiolated SWCNTs/ AuNPs showed remarkable detection sensitivity with an estimated detection limit of 0.8 pM.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada; NRC Biotechnology Research Institute
Peer reviewedNo
NRC number49939
NPARC number12465493
Export citationExport as RIS
Report a correctionReport a correction
Record identifierdd837ce9-b257-4968-8029-a81522855e51
Record created2009-10-26
Record modified2016-05-09
Bookmark and share
  • Share this page with Facebook (Opens in a new window)
  • Share this page with Twitter (Opens in a new window)
  • Share this page with Google+ (Opens in a new window)
  • Share this page with Delicious (Opens in a new window)