Characterization of the type III secretion associated low calcium response genes of Vibrio parahaemolyticus RIMD2210633

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DOIResolve DOI: http://doi.org/10.1139/w2012-109
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TypeArticle
Journal titleCanadian Journal of Microbiology
ISSN0008-4166
Volume58
Issue11
Pages13061315; # of pages: 10
Subjecttype III secretion, calcium, effector, cytotoxicity
AbstractVcio parahaemolyticus T3SS-1 substrates have been identified, although many predicted substrates (based on homologies) remain undetected in secreted fractions and therefore uncharacterized. We have experimentally developed and optimized a secretion assay protocol allowing for reliable and reproducible detection of V. parahaemolyticus T3SS-1 secreted proteins within culture supernatants. The presence of magnesium and absence of calcium were critical factors in promoting type III secretion of protein substrates. Proteomic approaches identified known V. parahaemolyticus secreted effectors in addition to previously unidentified proteins. Isogenic mutants in putative low calcium response genes were generated, and experiments further implicated the genes in secretion and V. parahaemolyticus-mediated host-cell cytotoxicity during infection. These approaches should be valuable towards future detailed genetic and biochemical analyses of T3SS-1 in V. parahaemolyticus.
Publication date
LanguageEnglish
AffiliationAquatic and Crop Resource Development; National Research Council Canada
Peer reviewedYes
NPARC number21270064
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Record identifierdeb5eebb-8d48-4106-970f-29d723573cf8
Record created2013-12-18
Record modified2016-05-09
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