Small unilamellar vesicles : a platform technology for molecular imaging of brain tumors

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DOIResolve DOI: http://doi.org/10.1088/0957-4484/22/19/195102
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TypeArticle
Journal titleNanotechnology
Volume22
Issue19
AbstractMolecular imaging enables the non-invasive investigation of cellular and molecular processes. Although there are challenges to overcome, the development of targeted contrast agents to increase the sensitivity of molecular imaging techniques is essential for their clinical translation. In this study, spontaneously forming, small unilamellar vesicles (sULVs) (30 nm diameter) were used as a platform to build a bimodal (i.e., optical and magnetic resonance imaging (MRI)) targeted contrast agent for the molecular imaging of brain tumors. sULVs were loaded with a gadolinium (Gd) chelated lipid (Gd-DPTA-BOA), functionalized with targeting antibodies (anti-EGFR monoclonal and anti-IGFBP7 single domain), and incorporated a near infrared dye (Cy5.5). The resultant sULVs were characterized in vitro using small angle neutron scattering (SANS), phantom MRI and dynamic light scattering (DLS). Antibody targeted and nontargeted Gd loaded sULVs labeled with Cy5.5 were assessed in vivo in a brain tumor model in mice using time domain optical imaging and MRI. The results demonstrated that a spontaneously forming, nanosized ULVs loaded with a high payload of Gd can selectively target and image, using MR and optical imaging, brain tumor vessels when functionalized with anti-IGFBP7 single domain antibodies. The unique features of these targeted sULVs make them promising molecular MRI contrast agents.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada; NRC Genomics and Health Initiative; NRC Institute for Biological Sciences; NRC Canadian Neutron Beam Centre; NRC Institute for Biodiagnostics; NRC Institute for National Measurement Standards
Peer reviewedYes
NPARC number17451146
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Record identifierdf91d884-060c-436c-ab4b-820af0b8f8f5
Record created2011-03-30
Record modified2016-05-09
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