Comparison of five major trichome regulatory genes in Brassica villosa with orthologues within the Brassicaceae

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Journal titlePLoS ONE
Article numbere95877
Pages112; # of pages: 12
Subjectamino acid; plant RNA; vegetable protein; Arabidopsis; article; Brassica oleracea; Brassica villosa; Brassicaceae; controlled study; cotyledon; diploidy; DNA polymorphism; ENHANCER OF GLABRA gene; gene expression profiling; genetic variability; GLABRA 1 gene; GLABRA 2 gene; molecular cloning; molecular evolution; nonhuman; nucleotide sequence; orthology; plant development; plant gene; plant genome; plant leaf; prediction; regulator gene; RNA sequence; RNA transcription; TRANSPARENT TESTA GLABRA 1 gene; trichome; TRIPTYCHON gene
AbstractCoding sequences for major trichome regulatory genes, including the positive regulators GLABRA 1(GL1), GLABRA 2 (GL2), ENHANCER OF GLABRA 3 (EGL3), and TRANSPARENT TESTA GLABRA 1 (TTG1) and the negative regulator TRIPTYCHON (TRY), were cloned from wild Brassica villosa, which is characterized by dense trichome coverage over most of the plant. Transcript (FPKM) levels from RNA sequencing indicated much higher expression of the GL2 and TTG1 regulatory genes in B. villosa leaves compared with expression levels of GL1 and EGL3 genes in either B. villosa or the reference genome species, glabrous B. oleracea; however, cotyledon TTG1 expression was high in both species. RNA sequencing and Q-PCR also revealed an unusual expression pattern for the negative regulators TRY and CPC, which were much more highly expressed in trichomerich B. villosa leaves than in glabrous B. oleracea leaves and in glabrous cotyledons from both species. The B. villosa TRY expression pattern also contrasted with TRY expression patterns in two diploid Brassica species, and with the Arabidopsis model for expression of negative regulators of trichome development. Further unique sequence polymorphisms, protein characteristics, and gene evolution studies highlighted specific amino acids in GL1 and GL2 coding sequences that distinguished glabrous species from hairy species and several variants that were specific for each B. villosa gene. Positive selection was observed for GL1 between hairy and non-hairy plants, and as expected the origin of the four expressed positive trichome regulatory genes in B. villosa was predicted to be from B. oleracea. In particular the unpredicted expression patterns for TRY and CPC in B. villosa suggest additional characterization is needed to determine the function of the expanded families of trichome regulatory genes in more complex polyploid species within the Brassicaceae. © 2014 Nayidu et al.
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AffiliationAquatic and Crop Resource Development; National Research Council Canada
Peer reviewedYes
NPARC number21272959
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Record identifierdfc5402f-2519-4d72-a112-9b9cb91ace16
Record created2014-12-03
Record modified2016-05-09
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