Berberine and plant stanols synergistically inhibit cholesterol absorption in hamsters

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DOIResolve DOI: http://doi.org/10.1016/j.atherosclerosis.2009.08.050
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TypeArticle
Journal titleAtherosclerosis
Volume209
Issue1
Pages111117; # of pages: 7
SubjectBerberine; plant stanols; plasma lipids; cholesterol absorption; cholesterol synthesis; LDL-receptor; hamster
AbstractThe present study was conducted to determine the efficacy and underlying mechanism of berberine (BBR), plant stanols (PS) and their combination on plasma lipids. Male Golden Syrian hamsters were randomly divided into 4 groups (n = 15/group) and fed a cornstarch–casein–sucrose-based diet containing 0.15 percent cholesterol and 5 percent fat. Three treatment groups were supplemented with 0.17 percent (equivalent to 100 mg kg−1 d−1) BBR, 1 percent PS, or a combination of both (BBRPS) for 4 wk. At the end of the study, plasma lipids were analyzed with enzymatic methods, cholesterol absorption and synthesis using stable isotope tracer methodology, and gene and protein expressions in the liver and small intestine using real-time PCR and Western blot, respectively. BBR and PS significantly lowered plasma total- and nonHDL-cholesterol levels, and BBRPS markedly improved cholesterol-lowering efficacy compared to BBR or PS alone. Further examinations revealed that BBR and PS both inhibited cholesterol absorption and by contrast, increased cholesterol synthesis, and exerted a synergistic action when they were combined. Plasma total or nonHDL-cholesterol levels were significantly correlated with cholesterol absorption rates. BBR upregulated sterol 27-hydroxlase gene expression and BBRPS increased both cholesterol-7α-hydroxylase and sterol 27-hydroxlase gene expressions. BBR and PS also synergistically decreased plasma triacylglycerides. These findings suggest that the cholesterol-lowering action of BBR might involve a combination of inhibition of cholesterol absorption and stimulation of bile acid synthesis. The combination of BBR and PS improves cholesterol-lowering efficacy through a synergistic action on cholesterol absorption, in addition to synergistically reducing plasma triacylglycerols in hamsters.
Publication date
LanguageEnglish
AffiliationNRC Institute for Nutrisciences and Health; National Research Council Canada
Peer reviewedYes
NPARC number20097242
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Record identifiere0df9183-7324-40de-aab4-14ff12d26b78
Record created2012-06-07
Record modified2016-05-09
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