Cleavage of Supercoiled DNA by Horseradish Peroxidase plus tert-Butyl Hydroperoxide Is Not Due to tert-Butylperoxyl Radicals

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TypeArticle
Journal titleChemical Research in Toxicology
ISSN0893228X
Volume15
Issue10
Pages13241329; # of pages: 6
AbstractTwo supercoiled (SC), double-stranded DNAs, pBR 322 and pUC 19, have been subjected to oxidative stress using horseradish peroxidase (HRP) and HRP + tert-butyl hydroperoxide (BOOH). HRP alone causes single-strand cleavage of these SC DNAs. Strand cleavage is enhanced substantially in the presence of commercial BOOH (which contains H2O2) but is, at best, only very slightly enhanced in the presence of pure BOOH. In the HRP/pure BOOH system, the DNA single-strand-scission which does occur appears to be due to a direct action of oxidized HRP. It is not due to tert-butylperoxyl radicals because strand-scision is not even retarded by 10 mM Trolox, an outstanding water-soluble trap for peroxyl radicals. The present results are congruent with our earlier conclusion [Paul, T., et al. (2000) Biochemistry 39, 4129] that neutral alkylperoxyl radicals produce little or no direct single-strand-scission in SC DNAs.
Publication date
Linkhttp://pubs3.acs.org/acs/journals/doilookup?in_doi=10.1021/tx020043o
AffiliationNational Research Council Canada; NRC Steacie Institute for Molecular Sciences
Peer reviewedNo
Identifier10439298
NPARC number12333569
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Record identifiere167eb9d-85b3-42d9-829e-76673f36e723
Record created2009-09-10
Record modified2016-05-09
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