Determination of erythromycin A by liquid chromatography and electrochemical detection, with application to salmon tissue

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DOIResolve DOI: http://doi.org/10.1016/0378-4347(93)80446-B
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TypeArticle
Journal titleJournal of Chromatography B: Biomedical Sciences and Applications
ISSN0378-4347
Volume619
Issue1
Pages6369
AbstractThe chromatographic performance of erythromycin A (EA) is improved significantly over that achieved on polymeric columns by using a sterically shielded octyldiisopropylsilica (Zorbax Rx-C8) column and a neutral mobile phase consisting of 5 mM aqueous sodium perchlorate—acetonitrile (50:50). This mobile phase facilitates electrochemical detection of EA at the 3-pmol level. Temperature control of both column and detection cell is important for minimizing detector noise and drift. A clean-up procedure, based on aminopropylsilica solid-phase extraction, allows the detection of EA in salmon flesh down to the 0.2-ppm level. Some of the metabolites of EA that retain the tertiary amine may also be detected by this method.
Publication date
LanguageEnglish
AffiliationNRC Institute for Marine Biosciences; National Research Council Canada
Peer reviewedYes
NPARC number23001019
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Record identifiere16ee5b1-84b3-478a-886e-6d9991d23d12
Record created2016-11-29
Record modified2016-12-16
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