Photo-uncaging of ceramides promotes reoganizatoin of liquid-ordered domains in supported lipid bilayers

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DOIResolve DOI: http://doi.org/10.1021/la3039158
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TypeArticle
Journal titleLangmuir
Volume29
Issue10
Pages33803387; # of pages: 8
Abstract6-Bromo-7-hydroxycoumarin (Bhc)-caged ceramide (Cer) analogs were incorporated into supported lipid bilayers containing a mixture of coexisting liquid-ordered (Lo) and liquid-disordered (Ld) phases. The release of N-palmitoyl and N-butanoyl-D-erythro-sphingosine (C16- and C4-Cer) by the photolysis of caged Cers using long-wavelength UV light was studied using a combination of atomic force microscopy and fluorescence microscopy. This approach demonstrated the ability to generate Cer with spatial and temporal control, providing an alternative method to the enzymatic generation of Cer. The generation of C16-Cer from Bhc-C16-Cer disrupted the Lo domains, with the incorporation of small fluid-phase regions and the disappearance of some smaller domains. Cer-rich gel-phase domains were not observed, in contrast to results reported by either direct Cer incorporation or enzymatic Cer generation. The photorelease of C4-Cer from Bhc-C4-Cer resulted in qualitatively similar changes in bilayer morphology, with the disappearance of some Lo domains and no evidence of Cer-rich gel domains but with a smaller height difference between the ordered and disordered phases.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada; NRC Steacie Institute for Molecular Sciences; Measurement Science and Standards
Peer reviewedYes
NPARC number21268890
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Record identifiere19346c1-d4a9-4fe2-b284-f46279c222f6
Record created2013-11-22
Record modified2016-05-09
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