Thio-glucose bound gold nanoparticles enhance radio-cytotoxic targeting of ovarian cancer

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DOIResolve DOI: http://doi.org/10.1088/0957-4484/22/28/285101
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TypeArticle
Journal titleNanotechnology
ISSN0957-4484
Volume22
Issue28
Article number285101
SubjectAnnexins; Cell apoptosis; Gold Nanoparticles; Intracellular uptake; Ovarian cancers; Reactive oxygen species; Therapeutic efficiency; Atomic emission spectroscopy; Atomic spectroscopy; Radiotherapy; metal nanoparticle; reactive oxygen metabolite
AbstractThe treatment of ovarian cancer has traditionally been intractable, and required novel approaches to improve therapeutic efficiency. This paper reports that thio-glucose bound gold nanoparticles (Glu-GNPs) can be used as a sensitizer to enhance ovarian cancer radiotherapy. The human ovarian cancer cells, SK-OV-3, were treated by gold nanoparticles (GNPs) alone, irradiation alone, or GNPs in addition to irradiation. Cell uptake was assayed using inductively coupled plasma atomic emission spectroscopy (ICP-AES), while cytotoxicity induced by radiotherapy was measured using both 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di-phenytetrazoliumromide and clonogenic assays. The presence of reactive oxygen species (ROS) was determined using CM-H2-DCFDA confocal microscopy and cell apoptosis was determined by an Annexin V-FITC/propidium iodide (PI) kit with flow cytometry. The cells treated by Glu-GNPs resulted in an approximate 31% increase in nanoparticle uptake compared to naked GNPs (p < 0.005). Compared to the irradiation alone treatment, the intracellular uptake of Glu-GNPs resulted in increased inhibition of cell proliferation by 30.48% for 90kVp and 26.88% for 6MV irradiation. The interaction of x-ray radiation with GNPs induced elevated levels of ROS production, which is one of the mechanisms by which GNPs can enhance radiotherapy on ovarian cancer. © 2011 IOP Publishing Ltd.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); National Institute for Nanotechnology
Peer reviewedYes
NPARC number21272048
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Record identifiere202e357-eb1e-4f85-ae19-4ae66839934b
Record created2014-05-27
Record modified2016-05-09
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