Titania–hydroxyapatite nanocomposite coatings support human mesenchymal stem cells osteogenic differentiation

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DOIResolve DOI: http://doi.org/10.1002/jbm.a.32964
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TypeArticle
Journal titleJournal of Biomedical Materials Research Part A
SubjectMesenchymal stem cells (MSCs); osteogenic differentiation in vitro; nanostructured titania-hydroxyapatite (TiO2– HA) coatings
AbstractIn addition to mechanical and chemical stability, the third design goal of the ideal bone-implant coating is the ability to support osteogenic differentiation of mesenchymal stem cells (MSCs). Plasma-sprayed TiO ₂-based bone-implant coatings exhibit excellent long-termmechanical properties, but their applications in bone implants are limited by their bioinertness. We have successfully produced a TiO ₂ nanostructured (grain size <50 nm) based coating charged with 10% wt hydroxyapatite (TiO ₂–HA) sprayed by high-velocity oxy-fuel. On Ti64 substrates, the novel TiO ₂–HA coating bond 153x stronger and has a cohesive strength 4x higher than HA coatings. The HA microand nano-sized particles covering the TiO ₂–HA coating surface are chemically bound to the TiO2 coating matrix, producing chemically stable coatings under high mechanical solicitations. In this study, we elucidated the TiO ₂–HA nanocomposite coating surface chemistry, and in vitro osteoinductive potential by culturing human MSCs (hMSCs) in basal and in osteogenic medium (hMSC-ob). We assessed the following hMSCs and hMSC-ob parameters over a 3-week period: (i) proliferation; (ii) cytoskeleton organization and cell–substrate adhesion; (iii) coating– cellular interaction morphology and growth; and (iv) cellular mineralization. The TiO2–HA nanocomposite coatings demonstrated 3x higher hydrophilicity than HA coatings, a TiO2-nanostructured surface in addition to the chemically bound HA micron- and nano-sized rod to the surface. hMSCs and hMSC-ob demonstrated increased proliferation and osteoblastic differentiation on the nanostructured TiO ₂–HA coatings, suggesting the TiO ₂–HA coatings nanostructure surface properties induce osteogenic differentiation of hMSC and support hMSC-ob osteogenic potential better than our current golden standard HA coating.
Publication date
LanguageEnglish
AffiliationNRC Industrial Materials Institute; National Research Council Canada
Peer reviewedYes
NRC number53817
NPARC number18375257
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Record identifiere2c3010a-4141-4f6b-a99c-2c33416af781
Record created2011-08-04
Record modified2017-03-23
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