Structure of the capsular polysaccharide of Actinobacillus pleuropneumoniae serotype 5b

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TypeArticle
Journal titleEuropean Journal of Biochemistry
Volume204
Issue1
Pages225230; # of pages: 6
SubjectACID; Actinobacillus; Actinobacillus pleuropneumoniae; analysis; bacterial; Bacterial Capsules; Canada; capsular polysaccharide; capsule; Carbohydrate Conformation; Carbohydrate Sequence; chemistry; D-GLUCOSE; HETERONUCLEAR; homonuclear; Hydrolysis; KDO; MAGNETIC; Magnetic Resonance Spectroscopy; MAGNETIC-RESONANCE; Methylation; Methylation analysis; MOLECULAR; Molecular Sequence Data; Molecular Structure; Molecular Weight; NUCLEAR; Nuclear Magnetic Resonance; NUCLEAR-MAGNETIC-RESONANCE; Pleuropneumoniae; POLYSACCHARIDE; Polysaccharides; Polysaccharides,Bacterial; REPEATING UNIT; RESONANCE; SEROTYPE; STRAIN; structure; TRISACCHARIDE; UNIT
AbstractThe capsular polysaccharide of Actinobacillus pleuropneumoniae serotype 5b (strain L20) was found to be a high molecular mass polymer composed of 2-acetamido-2-deoxy-D-glucose, D-glucose, and 3-deoxy-D-manno-octulosonic acid (KDO). Methylation analysis, partial hydrolysis and a combination of homonuclear and 1H-detected heteronuclear shift-correlated nuclear magnetic resonance experiments showed the polysaccharide to be a branched polymer of a trisaccharide repeating unit, having the structure: [formula; see text]
Publication date
LanguageEnglish
AffiliationNRC Institute for Biological Sciences; National Research Council Canada
Peer reviewedYes
NRC numberALTMAN1992A
NPARC number9364204
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Record identifiere3447801-6d81-4088-a245-b7cee7240dfb
Record created2009-07-10
Record modified2016-06-01
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